Light detection, autoradiography, and color changes are used as detection methods in Southern blotting. The labeling methods used in Western blotting are the use of fluorescently or radiolabelled antibodies, chromogenic dyes or diaminobenzidine formation, etc. No step like the blockade is involved in the transfer of the south.ĭuring Western blotting, nonspecific antibody sites are blocked on nitrocellulose paper with the help of powdered milk or bovine serum albumin (BSA).Ĭommon labeling methods used in southern blotting are the use of chromogenic dyes or radioactive or fluorescent labeling, etc.
Western Blot follows the electrical blot procedure.ĭuring Southern blotting, the sample must be denatured.ĭuring the immunoblot, the sample must be in its original state. Southern blotting follows the capillary blot procedure. SDS PAGE / Polyacrylamide gel used to separate proteins in Western Blot Primary and secondary antibodies are used as a probe.Īgarose gel electrophoresis is used for Southern blotting. Single-stranded DNA or sometimes RNA is used as a probe. It works on the principle of the immunodetection method or the antigen-antibody interaction. It works on the principle of hybridization. Western blotting is used to discover a specific protein or amino acid sequence. Southern blotting is used to discover a specific DNA sequence. Western Blot was developed by George Stark’s group at Stanford University in 1979. Southern blotting was developed by Edward M. Comparative chart Southern blottingĪ technique used to discover a specific DNA sequence in a given mixture is known as southern blot.Ī technique that is used to discover a specific protein amino acid sequence in a given mixture is known as southern blot. This name was used to coincide with the Southern Blot. Southern blotting is used to find a specific DNA sequence, while Western blotting is used to find a specific protein or amino acid sequence. Hence, it is known as southern transfer. On the other hand, the Western Blot was developed by George Stark’s group at Stanford University in 1979. Southern blot is the type of blot used to detect DNA, while Western blot it is used to discover proteins. The southern transfer was developed by Edward M. Southern in 1975. southern blot, northern blot, and western blot. Western Transferīlotting is a standard technique that scientists use to separate different molecules from a mixture or sample. In this technique, macromolecules such as nucleic acids (DNA and RNA) and proteins in a mixture are moved through a gel plate. Here, tiny particles will move faster than larger ones. These molecules are pressed against the surface of an immobilized membrane which transfers the molecules to the membrane. There are different types of blot based on the detection of specific material, i.e. The main difference between Southern Blot and Western Blot is that Southern Blot is a technique used to detect a specific DNA fragment in a given sample, while Western Blot is used to find a specific protein in a given sample.
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